Methods for the absolute quantification of N-glycan biomarkers.

摘要: Abstract Background Many treatment options especially for cancer show a low efficacy the majority of patients demanding improved biomarker panels patient stratification. Changes in glycosylation are hallmark many cancers and inflammatory diseases great potential as clinical disease markers. The large inter-subject variability due to hereditary environmental factors can complicate rapid transfer glycan markers into practice but also presents an opportunity personalized medicine. Scope review This discusses opportunities biomarkers medicine reviews methodology N-glycan analysis with specific focus on methods absolute quantification. Major conclusions entry is delayed part lack adequate precise robust quantification protein glycosylation. Only provide complete picture related changes will method robustness required by applications. General significance Glycan have huge use stable isotope labeled glycans internal standards heavy-isotope labeling necessary precision acceptable use. article Special Issue entitled “Glycans medicine” Guest Editor: Professor Gordan Lauc.