Skeletal muscle cell contraction reduces a novel myokine, chemokine (C-X-C motif) ligand 10 (CXCL10): potential roles in exercise-regulated angiogenesis

摘要: Accumulating evidence indicates that skeletal muscle secrets proteins referred to as myokines and exercise contributes their regulation. In this study, we propose chemokine (C-X-C motif) ligand 10 (CXCL10) functions a novel myokine. Initially, stimulated differentiated C2C12 myotubes with or without electrical pulse stimulation (EPS) identify myokines. Cytokine array analysis revealed CXCL10 secretion was significantly reduced by EPS, which further confirmed enzyme-linked immunosorbent assay quantitative polymerase chain reaction analysis. Treadmill experiments in mice identified significant reduction of Cxcl10 gene expression the soleus muscle. Additionally, contraction-dependent p38 MAPK activation appeared be involved reduction. Furthermore, conditioned medium obtained after applying EPS could induce survival MSS31, vascular endothelial cell model, partially attenuated addition recombinant CXCL10. Overall, our findings suggest exercise-reducible myokine, control viability.