Molekularbiologische Untersuchungen zu zentralnervösen Alterungsprozessen der Reproduktionsfunktion in der weiblichen Ratte

摘要: GABA neurones, as part of the GnRH network, play a significant role in changes age-related processes reproduction function. In order to study regulation ovarian steroids on expression GAD associated regions male rat-model has been chosen first this study. Firstly, endocrine status animals determined through method radioimmunoassay (RIA). Using situ hybridisation preoptic region anterior hypothalamus (POA), suprachiasmatis nucleus (SCN), medio basel (MBH), and dentate gyrus cellular gene transcripts both isoforms, GAD65 GAD67, were measured. all these no effects castration followed by recovery period three weeks could be monitored. The GAD67 POA was enhanced administration estradiol almost 40%, compared control group. However, other investigated displayed detectible alterations. treatment with testosterone effected only: A down 30 % 15 CSN. decreased MBH gyrus. results suggest that regulate different manner consequently also inhibitory functions GABA. As an effect unlike measured SCN, it concluded, androgens have their own pathway GAD. fact increases is accompanied decrease LH concentration serum group confirms hypothesis activities act transmit positive feedback release at level und not axons. progesterone may cause peak persistent estrous rats reinstate regular cycle. Accordingly animal-model developed second investigate molecular biological age-dependend changes. Three-months-old proestrous (Y) 12 months-old (MA) used model. Unlike Y-rats MA-rats treated progesteron. sacrificed 13 h 17 h. MA-group untreated animals, 10 considered value (5 ng/ml LH) set up according concentrations groups determine responding (responding animals). An induction surge Progesterone successfully 44 animals. addition, model analogy MA-13 Y-13 well Y-17 observed. Taqman®-PCR quantitative competitive RT-PCR obtain clear statistical along hypothalamic-pituitary-ovarian axis individual following genes examined study: ER α β, GnRH, GnRH-R, FSH-β. Age-related detected POA, pituitary ovary: β analogue (34 %), (44 %) ovary (almost 30%). pituitary, mRNA exhibited enhancement 55 middle-aged 153 young FSH-β elevated likewise 300 occurred suggests are expressed aged-dependently involved causally remaining age-relevant axis. Since ovary, lead main conclusion, essential for maintenance study, highly satisfactory profit reinstatement cycle examinations This furthermore allowed examination relatively high number