Processing of gonadotropin-releasing hormone gene transcripts in the rat brain.

摘要: Abstract The precursor of gonadotropin-releasing hormone (GnRH) and the 56-amino acid GnRH-associated peptide is encoded in an mRNA about 560 bases length. This derives from approximately 4300-base pair-long gene consisting four relatively short exons (denoted 1, 2, 3, 4) three large introns (A, B, C). In this study, we characterized order by which are spliced primary transcript processing intermediates to give rise a mature evaluated potential role transcription pre-mRNA control proGnRH levels vivo. Nuclear cytoplasmic RNA fractions isolated rat preoptic area-anterior hypothalamus (POA-AH) basal olfactory area (located rostral POA) were analyzed 1) solution hybridization-RNase protection mapping using several probes directed at various regions 2) reverse transcription-polymerase chain reaction oligonucleotide primers. Both types analysis showed that begins with splicing intron B transcript. Hence, ideal target for studying situ hybridization. Subsequent A C appeared take place two alternative, although not equally prevalent pathways. Quantitative indicated hnRNA species constituted, on mole basis, 20% total transcripts POA-AH. alone constituted 10% POA-AH as much area. prospect blockade means hybridization endogenous antisense RNAs (transcribed SH opposite strand same DNA locus) did prove be likely, present very low compared any species. We conclude pool may reflect high rate and/or slow processing.