Description of a multiplex Bordetella pertussis and Bordetella parapertussis LightCycler® PCR assay with inhibition control
作者： Joann L. Cloud , Weston C. Hymas , Arthur Turlak , Ann Croft , Udo Reischl
DOI: 10.1016/S0732-8893(03)00045-2
关键词:
摘要: While culture for Bordetella species is highly specific, sensitivity extremely variable due to patient age, immunization status, antibiotic treatment, and specimen transport conditions. We evaluated a real-time multiplex PCR assay as an alternative the detection differentiation of pertussis parapertussis. The conditions allowed simultaneous one B. organism five parapertussis organisms per reaction. An inhibition control was incorporated into assay. Of 163 total samples evaluated, 37 38 positive by either or direct fluorescent antibody testing (DFA) were also (97% sensitivity). 125 culture- DFA-negative samples, 101 negative (81% specificity). described rapid, sensitive, contamination-limiting, that controls inhibition. performs well using liquid swab from dried material on slides.
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Udo Reischl, Katrin Kösters, Birgit Leppmeier, Hans-Jörg Linde, Norbert Lehn, Rapid Detection and Simultaneous Differentiation of Bordetella pertussis and Bordetella parapertussis in Clinical Specimens by LightCycler PCR Rapid Cycle Real-Time PCR — Methods and Applications. pp. 31- 43 ,(2002) , 10.1007/978-3-642-48351-6_3
S Smith, R C Tilton, Acute Bordetella pertussis infection in an adult. Journal of Clinical Microbiology. ,vol. 34, pp. 429- 430 ,(1996) , 10.1128/JCM.34.2.429-430.1996
R M Wadowsky, S Laus, T Libert, S J States, G D Ehrlich, Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab. Journal of Clinical Microbiology. ,vol. 32, pp. 1054- 1057 ,(1994) , 10.1128/JCM.32.4.1054-1057.1994
Mary J. R. Gilchrist, Karla S. Long, Mike J. Loeffelholz, Curt J. Thompson, Detection of Bordetella holmesii using Bordetella pertussis IS481 PCR assay. Journal of Clinical Microbiology. ,vol. 38, pp. 467- 467 ,(2000) , 10.1128/JCM.38.1.467-467.2000
Mike J. Loeffelholz, Curt J. Thompson, Karla S. Long, Mary J. R. Gilchrist, Comparison of PCR, culture, and direct fluorescent-antibody testing for detection of Bordetella pertussis. Journal of Clinical Microbiology. ,vol. 37, pp. 2872- 2876 ,(1999) , 10.1128/JCM.37.9.2872-2876.1999
Seth W Wright, Kathryn M Edwards, Michael D Decker, Marie H Zeldin, Pertussis Infection in Adults With Persistent Cough JAMA: The Journal of the American Medical Association. ,vol. 273, pp. 1044- 1046 ,(1995) , 10.1001/JAMA.1995.03520370086042
Peter A.G Tilley, M.V Kanchana, Ineke Knight, Joseph Blondeau, Nick Antonishyn, Harry Deneer, Detection of Bordetella pertussis in a clinical laboratory by culture, polymerase chain reaction, and direct fluorescent antibody staining; accuracy, and cost Diagnostic Microbiology and Infectious Disease. ,vol. 37, pp. 17- 23 ,(2000) , 10.1016/S0732-8893(00)00117-6
J. G. Deville, J. D. Cherry, P. D. Christenson, E. Pineda, C. T. Leach, T. L. Kuhls, S. Viker, Frequency of Unrecognized Bordetella pertussis Infections in Adults Clinical Infectious Diseases. ,vol. 21, pp. 639- 642 ,(1995) , 10.1093/CLINIDS/21.3.639
U. Heininger, K. Stehr, J. D. Cherry, Serious pertussis overlooked in infants European Journal of Pediatrics. ,vol. 151, pp. 342- 343 ,(1992) , 10.1007/BF02113254
L. A. Cattaneo, G. W. Reed, D. H. Haase, M. J. Wills, K. M. Edwards, The Seroepidemiology of Bordetella pertussis Infections: A Study of Persons Ages 1–65 Years The Journal of Infectious Diseases. ,vol. 173, pp. 1256- 1259 ,(1996) , 10.1093/INFDIS/173.5.1256