Arginase-1: A novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology †‡§

摘要: BACKGROUND: Arginase-I is a key urea cycle metalloenzyme that has been used as an immunohistochemistry (IHC) marker for hepatocellular carcinoma (HCC). Previous studies have demonstrated the efficacy of HepPar-1 and glypican-3 (GPC-3) IHC in liver fine needle aspiration (FNA) cytology. METHODS: Arginase-1 was performed on FNA cell blocks, its performance characteristics were compared with GPC-3. Ninety-two formalin-fixed, paraffin-embedded blocks selected (HCC [n = 44], cirrhosis 2], focal nodular hyperplasia 3], hepatic adenomas dysplastic nodules 6], metastatic carcinomas 35]). staining antibodies directed against arginase-1, HepPar-1, GPC-3 appropriate positive negative controls. RESULTS: Arginase-1 positivity 37 44 (84.1%) cases HCC, 32 (72.7%) 25 (56.8%) GPC-3, respectively. Arginase-1 expression not observed any (0%), whereas present 1 case carcinoma. In addition, both arginase-1 all 13 (100%) nonmalignant lesions, absent (0%). CONCLUSION: This study demonstrates are effective markers differentiation. Furthermore, superior sensitivity diagnosis specificity, benign lesions. Hence, use can aid HCC separating from Cancer (Cancer Cytopathol) 2012. © 2012 American Society.