Analysis of integrin expression on oligodendrocytes during axo-glial interaction by using rat-mouse xenocultures

摘要: To analyze the expression of cell-surface molecules on neurons or glia during myelination, we have developed a xenotypic coculture system in which mouse oligodendrocytes interact with rat dorsal root ganglion neurons. The axo-glial interactions these cultures promote oligodendrocyte precursor cell proliferation, survival, and differentiation as vivo, thus supporting validity xenocultures model to study myelination can be distinguished using species- specific antibodies. We examined integrins, major family extracellular matrix receptors, early stages found that, unlike Schwann cells, do not express alpha 6 beta 4 association myelin sheath formation. pattern integrin observed is similar that seen differentiate purified cultures, it comprises 1, v 5, an yet uncharacterized v-associated subunit 80 kDa. Changes associated differentiation, therefore, depend axonal contact, required for formation, although its may contribute individual properties cells.