A role in migration for the alpha V beta 1 integrin expressed on oligodendrocyte precursors

作者: Richard Milner , Gwynneth Edwards , Charles Streuli , Charles ffrench-Constant

DOI: 10.1523/JNEUROSCI.16-22-07240.1996

关键词: Cell migrationIntegrinMigration AssayAlpha-v beta-5OligodendrocyteDownregulation and upregulationCell biologyBiologyAlpha-v beta-3Molecular biologyMonoclonal antibody

摘要: Myelination of the CNS requires migration oligodendrocyte precursors throughout from restricted regions within ventricular and subventricular zones. In light significant effects cell-extracellular matrix (ECM) interactions on cell in other developing systems, we have analyzed role integrins precursor migration. We shown previously that vitro express a limited repertoire integrins, including alpha 6 beta 1, v 3, differentiation is associated with downregulation 1 upregulation 5. Using assay based movement cells away an agarose drop containing high-density suspension, find RGD peptides (that block but not integrins) anti-beta antibodies astrocyte-derived ECM, whereas 3 little effect. These results suggest plays migration, this confirmed by use blocking monoclonal distinguish these two integrins. keeping others, differentiated oligodendrocytes lose migratory potential timing loss correlates 1. Taken together work others showing ECM ligands for are expressed CNS, propose integrin vivo its during could be important factor regulating phenotype cells.

参考文章(63)
J. Varani, W. Orr, P. A. Ward, A comparison of the migration patterns of normal and malignant cells in two assay systems. American Journal of Pathology. ,vol. 90, pp. 159- 172 ,(1978)
CE Shaw, R Milner, AS Compston, C ffrench-Constant, Analysis of integrin expression on oligodendrocytes during axo-glial interaction by using rat-mouse xenocultures The Journal of Neuroscience. ,vol. 16, pp. 1163- 1172 ,(1996) , 10.1523/JNEUROSCI.16-03-01163.1996
E Koivunen, D.A. Gay, E Ruoslahti, Selection of peptides binding to the alpha 5 beta 1 integrin from phage display library. Journal of Biological Chemistry. ,vol. 268, pp. 20205- 20210 ,(1993) , 10.1016/S0021-9258(20)80715-7
B E Vogel, G Tarone, F G Giancotti, J Gailit, E Ruoslahti, A novel fibronectin receptor with an unexpected subunit composition (alpha v beta 1). Journal of Biological Chemistry. ,vol. 265, pp. 5934- 5937 ,(1990) , 10.1016/S0021-9258(19)39268-3
A. Sonnenberg, H. Janssen, F. Hogervorst, J. Calafat, J. Hilgers, A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody. Journal of Biological Chemistry. ,vol. 262, pp. 10376- 10383 ,(1987) , 10.1016/S0021-9258(18)61123-8
Steven M. Albelda, Laszlo Damjanovich, Meenhard Herlyn, Clayton A. Buck, David E. Elder, Stephen A. Mette, RoseMary Stewart, Integrin Distribution in Malignant Melanoma: Association of the β3 Subunit with Tumor Progression Cancer Research. ,vol. 50, pp. 6757- 6764 ,(1990)