作者: Richard Milner , Gwynneth Edwards , Charles Streuli , Charles ffrench-Constant
DOI: 10.1523/JNEUROSCI.16-22-07240.1996
关键词: Cell migration 、 Integrin 、 Migration Assay 、 Alpha-v beta-5 、 Oligodendrocyte 、 Downregulation and upregulation 、 Cell biology 、 Biology 、 Alpha-v beta-3 、 Molecular biology 、 Monoclonal antibody
摘要: Myelination of the CNS requires migration oligodendrocyte precursors throughout from restricted regions within ventricular and subventricular zones. In light significant effects cell-extracellular matrix (ECM) interactions on cell in other developing systems, we have analyzed role integrins precursor migration. We shown previously that vitro express a limited repertoire integrins, including alpha 6 beta 1, v 3, differentiation is associated with downregulation 1 upregulation 5. Using assay based movement cells away an agarose drop containing high-density suspension, find RGD peptides (that block but not integrins) anti-beta antibodies astrocyte-derived ECM, whereas 3 little effect. These results suggest plays migration, this confirmed by use blocking monoclonal distinguish these two integrins. keeping others, differentiated oligodendrocytes lose migratory potential timing loss correlates 1. Taken together work others showing ECM ligands for are expressed CNS, propose integrin vivo its during could be important factor regulating phenotype cells.