A Ketone Ester Diet Increases Brain Malonyl-CoA and Uncoupling Proteins 4 and 5 while Decreasing Food Intake in the Normal Wistar Rat

摘要: Three groups of male Wistar rats were pair fed NIH-31 diets for 14 days to which added 30% calories as corn starch, palm oil, or R-3-hydroxybutyrate-R-1,3-butanediol monoester (3HB-BD ester). On the 14th day, animal brains removed by freeze-blowing, and brain metabolites measured. Animals ketone ester diet had elevated mean blood bodies 3.5 mm lowered plasma glucose, insulin, leptin. Despite decreased leptin, feeding ad lib voluntary food intake 2-fold 6 while malonyl-CoA was increased about 25% in ketone-fed group but not oil group. Unlike acute effects body metabolism perfused working heart, there no reduction free mitochondrial [NAD+]/[NADH] ratio nor energy ATP hydrolysis, compatible with observed 1.5-fold increase uncoupling proteins 4 5. Feeding supplemented l-glutamate 15–20% GABA 34% supporting view that fatty acids well can be metabolized brain.