Virally inactivated human platelet concentrate lysate induces regulatory T cells and immunosuppressive effect in a murine asthma model

摘要: Background Platelet concentrate lysates (PCLs) are increasingly used in regenerative medicine. We have developed a solvent/detergent (S/D)-treated PCL. The functional properties of this preparation should be unveiled. hypothesized that, due to transforming growth factor-β1 (TGF-β1) content, PCLs may exert immunosuppressive and anti-inflammatory functions. Study Design Methods PCL was prepared by S/D treatment, oil extraction, hydrophobic interaction chromatography. content TGF-β PCL determined enzyme-linked immunosorbent assay. Cultured CD4+ T cells were investigate the effects on expression transcription factor forkhead box P3 (Foxp3), inhibition T-cell proliferation, cytokine production. regulatory function PCL-converted analyzed suppressive BALB/c mice given before ovalbumin (OVA) sensitization challenge using an asthma model. Inflammatory parameters, such as level immunoglobulin E (IgE), airway hyperresponsiveness (AHR), bronchial lavage fluid eosinophils, cytokines assayed. Recombinant human (rHu) TGF-β1 control. Results PCL significantly enhanced development CD4+Foxp3+-induced (iTregs). Converted iTregs produced neither Th1 nor Th2 inhibited normal proliferation. PCL- rHuTGF-β-converted prevented OVA-induced asthma. rHuTGF-β-modified both reduced levels OVA-specific IgE AHR, eosinophilia, responses mice. Conclusion S/D-treated promotes Foxp3+ exerts properties. This finding help understand clinical platelet lysates.