Proteomic Investigation of Ram Spermatozoa and the Proteins Conferred by Seminal Plasma

作者: Taylor Pini , Tamara Leahy , Clement Soleilhavoup , Guillaume Tsikis , Valerie Labas

DOI: 10.1021/ACS.JPROTEOME.6B00530

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摘要: Sperm proteomes have emerged for several species; however, the extent of species similarity is unknown. Sheep are an important agricultural which a comprehensive sperm proteome has not been produced. In addition, potential proteomic factors from seminal plasma that may contribute to improved fertility after cervical insemination yet be explored. Here we use liquid chromatography–tandem mass spectrometry investigate ejaculated ram spermatozoa, with quantitative comparison epididymal spermatozoa. We also present published five other species. identified 685 proteins in most abundant involved metabolic pathways. Only 5% were detected species, suggest highly conserved structures and Of both 7% more two membrane-bound wer...

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