Combined use of chemotherapeutics and oncolytic adenovirus in treatment of AFP-expressing hepatocellular carcinoma.

摘要: BACKGROUND Hepatocellular carcinoma (HCC) which is always refractory to most chemotherapeutic agents may result in poor survival of patients with advanced HCC. Oncolytic adenovirus a new form for cancer gene therapy via its ability replicate and kill tumor cells tumor-specific manner. In order eradicate tumors effectively, the combination oncolytic has been considered. This study aimed systematically analyze possibility synergistic cytotoxicity adenoviruses agents. METHODS Several types human HCC cell lines were used determine specificity Ad5-HC Ad5-AFP (IRES) by measuring viability vitro antitumor efficiency vivo. The combined also assessed methyl thiazolyl tetrazolium assay. RESULTS Both significantly cytotoxic great 5-FU, doxorubicin, paclitaxel was synergistically effective killing cells. CONCLUSIONS These data suggest that sensitize chemotherapy an enhanced effect on