Fetal calf serum-primed dendritic cells induce a strong anti-fetal calf serum immune response and diabetes protection in the non-obese diabetic mouse.

摘要: In recent years, several investigators have shown that transfer of dendritic cells (DC) prevents diabetes development in non-obese diabetic (NOD) mice. Accumulating evidences showing DC cultured medium containing fetal calf serum (FCS) can induce a dominant unspecific immune response tumor models after i.v. injection prompted us to investigate if the protecting effect on NOD mice might be supported by induction an anti-FCS recipient Five-week-old were injected with FCS-cultured bone marrow-derived or PBS as control. Levels and anti-bovine albumin (BSA) antibodies measured Anti-FCS cellular responses also analysed single using vitro proliferation splenocytes either RPMI supplemented FCS, AIMV-BSA autologous mouse BSA read out. prevented high titers anti-BSA detected all DC-injected Besides, isolated from proliferated vigorously presence bovine proteins contrast control but removing abrogated level those suggesting lymphocytes been primed against vivo injection. All together, our data show induced humoral protective relies their immunostimulatory effects.