Human glioma cell culture: two FCS-free media could be recommended for clinical use in immunotherapy

摘要: Immunotherapy, particularly active vaccination, may be developed as an effective and safe treatment modality for malignant gliomas, which continue to have a poor prognosis, despite advances in surgical techniques adjuvant chemotherapy radiotherapy. Since no glioma-specific tumor-associated antigens (TAAs) been discovered, autologous tumor cells or well-established glioma cell lines could used future vaccination protocols induce antitumour immunity against unknown TAAs. One obstacle clinical use of these tumour vaccines is related foetal calf serum (FCS). Efforts are currently being directed toward developing FCS-free media serum-free alternatives culture vaccines. In this study, medium containing human one (UltraCulture), supplemented not with epidermal growth factor, were tested on morphology, survival, DNA content TAA expression biopsy primary cultures. Their effects compared FCS-containing medium. Results show that, whatever the used, significant variations morphology survival observed. Furthermore, serum-containing UltraCulture preserved at early passage cultures population interest present biopsies before culture. addition, profile eight TAAs was similar between media. These data indicate that promising candidates produce tumour-cell