Changes in the immunologic phenotype of human malignant glioma cells after passaging in vitro.

摘要: Although immunotherapeutic strategies against glioblastomas have been promising both in vitro and animal models, similar successes not realized human clinical trials. One reason may be that are based on prior studies primarily used glioblastoma cell lines passaged vitro, which accurately reflect the vivo properties of cells. In this report, we flow cytometry to quantify expression immunological surface molecules directly ex (prior any culturing) after varying passages vitro. Furthermore, ELISA quantitate cytokine secretion various We demonstrate culturing established led increases MHC class I ICAM-1 IL-6 TGF-beta(2). there were significant changes I, II, B7-2, ICAM-1, FasL when comparing tumor cells those a single passage After passaging once also TGF-beta(2) IL-10. This report indicates leads secreted cytokines, known affect ability immune initiate an anti-tumor response. These phenotype part explain limited success