Modulation of major histocompatibility complex Class I molecules and major histocompatibility complex—bound immunogenic peptides induced by interferon-α and interferon-γ treatment of human glioblastoma multiforme

摘要: Object Little is known about the quantitative modulation of major histocompatibility complex (MHC) Class I expression on human gliomas that effected by interferons; even less immunogenic peptides are accommodated in peptide-binding motifs MHC alleles these brain tumors. In this article authors investigated ability interferon (IFN)α and IFNγ to upregulate modulate acid-eluted I—bound glioblastoma multiforme (GBM) cells vitro. Methods Early-passage primary GBM cell cultures U87MG were incubated with varying doses INFα or ranging between 0 2000 U/ml. Upregulation was assayed immunocytochemical analysis, flow cytometry, Western blot analysis. Modulation from IFN-treated examined aid mass spectroscopy. The vitro molecule upregul...