Optimization of ultrasonic-assisted extraction of antioxidant compounds from blackberry leaves using response surface methodology

摘要: Abstract Response surface methodology was used to optimize experimental conditions for ultrasonic-assisted extraction of phenolic compounds from blackberry leaves. The Box-Behnken design (BBD) employed the optimization parameters in terms total phenolics and antioxidant capacity. optimal results ABTS CUPRAC were HCl concentration 0.41 0.45 M, methanol 61 64% (v/v), temperature 66 68 °C time 105 117 min, respectively. values agreed with those predicted within a 95% confidence level, thus indicating suitability response optimizing ultrasound-assisted showed that present leaves exhibited significant properties. Seven such as ellagic acid, caffeic chlorogenic quercetin, myricetin, kaempferol 3-β- d -glucopyranoside determined by HPLC-DAD after at optimum conditions.