The Impact of Lagging Strand Replication Mutations on the Stability of CAG Repeat Tracts in Yeast
作者： Shanda S. Reinke , Malia J. Ireland , Dennis M. Livingston
DOI: 10.1093/GENETICS/155.4.1657
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摘要: We have examined the stability of long tracts CAG repeats in yeast mutants defective enzymes suspected to be involved lagging strand replication. Alleles DNA ligase (cdc9-1 and cdc9-2) destabilize stable tract orientation, i.e., when serves as template. In this orientation nearly two-thirds events recorded cdc9-1 mutant were expansions. While neither allele significantly increases frequency tract-length changes unstable produced a significant number expansions orientation. A mutation primase (pri2-1) destabilizes both orientations. Mutations helicase/deoxyribonuclease (dna2-1) or two RNase H activities (rnh1Delta rnh35Delta) do not effect on repeat stability. interpret our results terms steps replication that are likely lead expansion contraction tracts.
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