The relationship of quantitative nuclear morphology to molecular genetic alterations in the adenoma-carcinoma sequence of the large bowel.

摘要: The relationship of abnormal nuclear morphology to molecular genetic alterations that are important in colorectal tumorigenesis is unknown. Therefore, Feulgen-stained isolated nuclei from 22 adenomas and 42 carcinomas had been analyzed for ras gene mutations allelic deletions on chromosomes 5q, 18q, 17p were characterized by computerized image analysis. Both area the shape factor representing irregularity correlated with adenoma-carcinoma progression (r = 0.57 r 0.52, P < 0.0001), whereas standard texture, a parameter chromatin homogeneity, was inversely -0.80, 0.0001). parameters strongly interrelated (P 0.0005). In multivariate analysis, predominantly associated or 0.0001) not influenced significantly individual alterations. Nuclear however, fractional loss, global measure changes, -0.39, 0.011). findings indicate neoplasms related tumor progression. biologic behavior appear be accumulated cancer-associated genes.