Nucleotide sequence and organization of the rat heme oxygenase gene.

摘要: Heme oxygenase, an essential enzyme of heme catabolism, is inducible by its substrate heme, heavy metals, and various other substances. To study the molecular mechanisms induction we isolated oxygenase gene from a rat genomic DNA library using cloned cDNA as hybridization probes determined complete nucleotide sequence. The composed 6830 nucleotides, organized in four introns five exons. transcription initiation site was identified S1 nuclease mapping analysis. Using HeLa cell lysate, confirmed that initiated accurately at assigned site. In 5'-flanking region gene, found several potential binding sites for different factors: factor Sp1, positive regulator control amino acid synthesis (GCN4), heat shock factor, metal-dependent factor. Furthermore, intron 1 contains sequence shows about 65% homology to neuronal identifier sequence, possible enhancer element.