The biological stimuli for governing the phase transition temperature of the "smart" polymer PNIPAM in water.
作者： Reddicherla Umapathi , P. Madhusudhana Reddy , Awanish Kumar , Pannuru Venkatesu , Chi-Jung Chang
DOI: 10.1016/J.COLSURFB.2015.08.020
关键词:
摘要: A lack of sufficient knowledge regarding the behaviour stimuli-responsive polymers to biological stimuli hinders potential use responsive as biomaterials and medical devices. Hence, in this study, we demonstrate impact various globular proteins on phase transition temperature poly(N-isopropylacrylamide) (PNIPAM) an aqueous solution through fluorescence spectroscopy, dynamic light scattering (DLS), Fourier transform infrared (FTIR) spectroscopy field-emission scanning electron microscopy (FESEM). Furthermore, describe molecular interaction PNIPAM with by MolDock method. Our experimental docking studies revealed that such α-chymotrypsin (CT), insulin (In) haemoglobin (Hb) decreased lower critical (LCST) polymer, whereas succinyl-concanavalin (SCA) increased LCST PNIPAM. The changed upon increasing concentration protein from 0.5mg/mL 1mg/mL. thermoresponsive can be significantly altered functional groups present protein. findings study used engineering bioresponsive smart PNIPAM-based
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