Three-dimensional cardiac tissue engineering using a thermoresponsive artificial extracellular matrix.

摘要: The purpose of this study was to try reconstitute three-dimensional cardiac tissue using a thermoresponsive artificial extracellular matrix, poly (N-isopropylacrylamide)-grafted gelatin (PNIPAM-gelatin), as the scaffold. PNIPAM-gelatin solution gels almost immediately when heated above 34 degrees C. We thought property could become advantageous scaffolding for reconstituting tissue. Because so quickly, all seeded cells in would entrapped and uniformly distributed toward three dimensions. Thus it be possible by very simple method mixing solution. Fetal rat were mixed with solution, incubated at 37 C allow mixture gel, cultured vitro. To define suitable culture conditions following parameters tested: (1) concentration, 0.04 approximately 0.125 mg/ml; (2) cell seeding density, 1 50 x 10(6) cells/ml; (3) addition or not hyaluronic acid. With concentration 0.05 mg/ml, density cells/ml, acid, reconstituted contracted synchronously. After hematoxylin eosin staining, tissue, cross-section 60 microm thick.