Three-dimensional engineered heart tissue from neonatal rat cardiac myocytes.

摘要: A technique is presented that allows neonatal rat cardiac myocytes to form spontaneously and coherently beating 3-dimensional engineered heart tissue (EHT) in vitro, either as a plane biconcaval matrix anchored at both sides on Velcro-coated silicone tubes or ring. Contractile activity was monitored standard organ baths continuously CO2 incubator for up 18 days (=26 after casting). Long-term measurements showed an increase force between 8 casting stable forces thereafter. At day 10, the twitch amplitude (TA) of electrically paced EHTs (average length × width thickness, 11 6 0.4 mm) 0.51 mN maximal development (Lmax) maximally effective calcium concentration. typical features heart: positive force–length negative force–frequency relation, high sensitivity (EC50 0.24 mM), modest inotropic (increase TA by 46%) pronounced lusitropic effect isoprenaline (decrease duration 21%). Both effects were sensitive muscarinic receptor agonist carbachol pertussis toxin-sensitive manner. Adenovirus-mediated gene transfer β-galactosidase into reached 100% efficiency. In summary, retain many physiological characteristics allow efficient with subsequent measurement. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 106–114, 2000.