Detection of bladder carcinoma by combined testing of urine for hyaluronidase and cytokeratin 20 RNAs

摘要: BACKGROUND A new, sensitive, noninvasive method for the detection of urothelial carcinomas urinary bladder would open new possibilities in both diagnosis and followup patients. METHODS This study included 228 patients diagnosed with carcinoma, 68 benign lesions, 44 healthy persons served as control group. All were subjected to: serologic schistosomiasis antibody assay serum, urine cytology, estimation hyaluronic acid (HA) by enzyme-linked immunosorbent assay, CK-20 hyaluronidase (HAase) reverse transcription polymerase chain reaction (RT-PCR) cells from voided urine. RESULTS HA mean rank was higher malignant groups than group (P < 0.0001) significantly related to tumor grade = 0.021). HA best-cutoff, determined using receiver operating characteristic curve discriminate between nonmalignant groups, 58.5 units/mg protein at 85.8% sensitivity 60.7% specificity. HAase RNA showed superior (90.8%) over cytology (68.9%) (78.1%) specificity 93.4%, 98.1% 80.2%, respectively. The reached 94.7% a 91.5% when combined CK-20. 4 investigated markers P <0.05. Whereas only stage 0.05). As schistosomiasis, positivity associated 0.038). CONCLUSIONS HAase is promising test high carcinoma detection. Cancer 2005. © 2005 American Society.