Exploring the Potential of Electrical Impedance Tomography for Tissue Engineering Applications

摘要: In tissue engineering, cells are generally cultured in biomaterials to generate three-dimensional artificial tissues repair or replace damaged parts and re-establish normal functions of the body. Characterizing cell growth viability these bioscaffolds is challenging, currently achieved by destructive end-point biological assays. this study, we explore potential use electrical impedance tomography (EIT) as a label-free non-destructive technology assess viability. The key challenge engineering application detect small change conductivity associated with sparse distributions regards size hosting scaffold, i.e., low volume fraction, until they assemble into larger tissue-like structure. We show proof-of-principle data, measure within both hydrogel microporous scaffold an ad-hoc EIT equipment, introduce frequency difference technique improve reconstruction.