Role of tumor necrosis factor-alpha in the ovulatory mechanism of ewes.

摘要: Tumor necrosis factor (TNF)-alpha was localized by indirect immunofluorescence microscopy to thecal endothelial cells or preovulatory ovine follicles. Immunostaining of within the apical hemisphere follicle declined abruptly with approach ovulation; counterpart basal follicular wall remained largely unaffected. It appeared that TNF alpha had been released into presumptive area rupture. Intrafollicular injection antiserum blocked ovulation in ewes. We considered possibility liberated from mediates programmed deletion ovarian surface and occurs formative ovulatory stigma sheep. Indeed, contiguous epithelium, endothelium, granulosal were protected apoptotic death (DNA fragmentation) exposure antibodies. Finally, induced cell apoptosis vitro. suggest a bioactive, soluble form generated follicles has an obligatory role biomechanics weakening