Microbial investigations in throat swab and tracheal aspirate specimens are beneficial to predict the corresponding endotracheal tube biofilm flora among intubated neonates with ventilator‑associated pneumonia

摘要: Ventilator-associated pneumonia (VAP) is a common nosocomial infection in neonatal intensive care units with high morbidity and mortality. Bacterial biofilm the endotracheal tube (ET) provides notable persistent source of pathogens that may cause VAP, thus important for VAP detection. However, during intubation microbial investigations ET, samples are unavailable due to infeasibility collecting ET neonates. It therefore great importance find alternative sources can help identify flora. In present study, signatures throat swabs tracheal aspirates were compared from neonates using 16S ribosomal RNA gene polymerase chain reaction, denaturing gradient gel electrophoresis (DGGE), cloning sequencing. Sequences assigned phylogenetic species BLAST. Microbial diversity richness among three types specimens based on their DGGE fingerprints, taxonomic characteristics BLAST results. The biofilms similar aspirate yet significantly different swab (P<0.05). Compared biofilms, overall constituent ratio microflora was However useful predicting Staphylococcus sp. sensitivity 85.7% specificity 83.3%. combination detect 100%. detection Pseudomonas assisted its identification (sensitivity 33.3% 100%). results study suggest beneficial identifying There be clinical applications substituent surveillance intubated