A new structure for the murine Xist gene and its relationship to chromosome choice/counting during X-chromosome inactivation.

摘要: In this report, we present structural data for the murine Xist gene. The presented in paper demonstrate that transcript is at least 17.4 kb, not 14.3 kb as previously reported. new structure of gene described herein has seven exons, six. Exon VII encodes an additional 3.1 information 3′ end. contains possible sites polyadenylation; four these are located newly discovered Consequently, it several distinct transcripts may be produced through differential polyadenylation a primary transcript. Alternative use signals could result size changes exon VII. Two major species detectable by Northern analysis, consistent with polyadenylation. paper, propose model role end process X-chromosome counting and choice during embryonic development.