Pretreatment of Addra gazelle (Nanger dama ruficollis) spermatozoa with cholesterol-loaded cyclodextrins improves cryosurvival

摘要: Abstract Preserving genetic diversity of the critically endangered Addra gazelle (Nanger dama ruficollis) could be enhanced through use frozen-thawed sperm and artificial insemination. Our aim was to characterize ejaculate traits assess effects cholesterol-loaded cyclodextrin (CLC) on cryosurvival. Fresh ejaculates were treated with CLC (0, 0.5, 1.5, 3.0, 6.0 mg/ml) prior cryopreservation. All males produced spermic >75% motility. The mean ± SEM seminal volume, concentration, percent motility, forward progression, morphologically normal spermatozoa 3.2 ± 0.3 ml, 1.2 ± 0.3 × 109, 75.82 ± 2.7%, 3.2 ± 0.3 (0–5 scale; 5 = most progressive), 57.12 ± 3.8%, respectively. More than 92% contained an intact acrosome. There no effect time or in vitro incubation progression acrosomal integrity thawed samples (P > 0.05). Spermatozoa pre-treated 0.5 mg/ml retained higher (P   0.05). Linearity (LIN) straightness (STR) different among after thawing. Results demonstrate treatment (0.5 mg/ml) protects from cryo-damage. Reported advances will facilitate establishment a frozen repository support management this north African desert antelope.