Protein Factor Obtained from Rat Adipose Tissue Specifically Permits the Proliferation of 3T3-L1 and OB1771 Preadipocyte Cell Lines in a Completely Defined Serum-Free Medium
作者： Teruo Kawada , Naohito Aoki , Etsuro Sugimoto
DOI: 10.1007/978-94-011-3550-4_22
关键词:
摘要: The proliferation of preadipocytes is essential for the formation new adipocytes and important to differentiation into adipocytes. In this study, we have first developed a completely defined serum-free medium that supports growth preadipocytes, then found novel protein factor, referred as PAGF (preadipocyte factor), in rat adipose tissue using medium. specifically permitted acting like competent its apparent molecular weight was about 20,000. activity existed various mammalian tissues should function response energy intake. These results strongly suggest may play key role by locally triggering contribute development tissue. provide useful tool further understanding obesity.
springer.com
sci-hub.st 参考文章(9)
G Pöll-Jordan, W Schmidt, G Löffler, Adipose conversion of 3T3-L1 cells in a serum-free culture system depends on epidermal growth factor, insulin-like growth factor I, corticosterone, and cyclic AMP. Journal of Biological Chemistry. ,vol. 265, pp. 15489- 15495 ,(1990) , 10.1016/S0021-9258(18)55422-3
P J Smith, L S Wise, R Berkowitz, C Wan, C S Rubin, Insulin-like growth factor-I is an essential regulator of the differentiation of 3T3-L1 adipocytes. Journal of Biological Chemistry. ,vol. 263, pp. 9402- 9408 ,(1988) , 10.1016/S0021-9258(19)76555-7
B J Klyde, J Hirsch, Increased cellular proliferation in adipose tissue of adult rats fed a high-fat diet. Journal of Lipid Research. ,vol. 20, pp. 705- 715 ,(1979) , 10.1016/S0022-2275(20)40024-0
T KAWADA, N AOKI, R KAWAI, E SUGIMOTO, Proliferation of 3T3-L1 preadipocytes in a completely defined serum-free medium. Cell Biology International Reports. ,vol. 14, pp. 567- 574 ,(1990) , 10.1016/0309-1651(90)91184-6
R. Negrel, P. Grimaldi, G. Ailhaud, Establishment of preadipocyte clonal line from epididymal fat pad of ob/ob mouse that responds to insulin and to lipolytic hormones Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 6054- 6058 ,(1978) , 10.1073/PNAS.75.12.6054
Naohito Aoki, Teruo Kawada, Taeko Umeyama, Etsuro Sugimoto, Protein factor obtained from rat adipose tissue specifically permits the proliferation of the 3T3-L1 and Ob1771 cell lines Biochemical and Biophysical Research Communications. ,vol. 171, pp. 905- 912 ,(1990) , 10.1016/0006-291X(90)91231-G
Isao Yamane, Mikio Kan, Hiroyoshi Hoshi, Yoshiki Minamoto, Primary culture of human diploid cells and its long-term transfer in a serum-free medium Experimental Cell Research. ,vol. 134, pp. 470- 474 ,(1981) , 10.1016/0014-4827(81)90448-1
Howard Green, Olaniyi Kehinde, None, Sublines of mouse 3T3 cells that accumulate lipid Cell. ,vol. 1, pp. 113- 116 ,(1974) , 10.1016/0092-8674(74)90126-3
K. Hanai, Y. Oomura, Y. Kai, K. Nishikawa, N. Shimizu, H. Morita, C. R. Plata-Salaman, Central action of acidic fibroblast growth factor in feeding regulation. American Journal of Physiology-regulatory Integrative and Comparative Physiology. ,vol. 256, ,(1989) , 10.1152/AJPREGU.1989.256.1.R217