Adipose conversion of 3T3-L1 cells in a serum-free culture system depends on epidermal growth factor, insulin-like growth factor I, corticosterone, and cyclic AMP.
作者： G Pöll-Jordan , W Schmidt , G Löffler
DOI: 10.1016/S0021-9258(18)55422-3
关键词:
摘要: A culture system for 3T3-L1 preadipocytes based on a serum-free chemically defined medium containing fetuin, transferrin, and pantothenate is described. In this system, adipose conversion depends the following conditions. 1) presence of high insulin concentrations (1 microM), addition corticosterone together with 1-methyl-3-isobutylxanthine (MIX) not more than first 4 days after confluence to induces maximal within 12-14 days. MIX may be replaced by forskolin or permeable analogues cAMP, indicating that its effect due elevated cellular cAMP levels. 2) At low nM), reduced. Growth hormone insulin-like growth factor I epidermal have present as supplement get conversion. 3) The induction in either accompanied post-confluent mitoses. Inhibitors DNA replication markedly reduce Fibroblast platelet-derived factor, although acting potent mitogens cells, do support induced MIX.
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