Subtractive hybridization--genetic takeaways and the search for meaning.

摘要: Gene expression profiling relies on mRNA extraction from defined cell systems, which in the case of pathological processes necessarily results use small quantities tissues, sometimes as little a few cells. This obviates many systems gene and is best carried out using cDNA amplified by poly(A) reverse transcription polymerase chain reaction, capable generating material representative all expressed genes samples one cell. Analysis this subtractive hybridization compares at different stages biological/pathological process allowing identification upregulated during process. The present not dependent their prior description or choice used screen such method ideal for identifying novel unsuspected genes. We have to identify involved normal osteoblastic differentiation Paget's disease bone it has been widely study number systems. method, its applications relationship with other methods are reviewed.