Linear polyethylenimine-graft-chitosan copolymers as efficient DNA/siRNA delivery vectors in vitro and in vivo
作者： Sushil K Tripathi , Ritu Goyal , Pradeep Kumar , Kailash C Gupta , None
DOI: 10.1016/J.NANO.2011.06.022
关键词:
摘要: Chitosan was partially converted to its chlorohydrin derivative by the reaction with epichlohydrin, which subsequently reacted varying amounts of lPEI(2.5 kD) obtain a series chitosan-lPEI(2.5 copolymers (CP). These were then characterized and evaluated in terms transfection efficiency (in vitro vivo), cell viability, DNA release buffering capacity. The CP-4 copolymer (the best among CP series) showed enhanced (-2 - 24 folds) comparison chitosan, kD), bPEI(25 Lipofectamine HEK293, HeLa CHO cells. capacity pH range 3 7.5), as shown confocal microscopy, DNA-release capability copolymers, found be significantly over chitosan. Intravenous administration CP-4/DNA polyplex mice followed reporter gene analysis highest expression spleen. Collectively, these results demonstrate potential safe efficient nonviral vector. From Clinical Editor: -PEI (2.5 (CP) their efficiency, studied. provided levels. method may used enhance transfection.
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