In situ protein-SIP highlights Burkholderiaceae as key players degrading toluene by para ring hydroxylation in a constructed wetland model

摘要: In constructed wetlands, organic pollutants are mainly degraded via microbial processes. Helophytes, plants that commonly used in these systems, provide oxygen and root exudates to the rhizosphere, stimulating degradation. While treatment performance of wetlands can be remarkable, a mechanistic understanding degradation processes rhizosphere is still limited. We investigated toluene removal wetland model system combining 16S rRNA gene sequencing, metaproteomics (13) C-toluene situ protein-based stable isotope probing (protein-SIP). The rhizospheric bacterial community was dominated by Burkholderiales Rhizobiales, each contributing about 20% total taxon abundance. Protein-SIP data revealed members Burkholderiaceae, proteins which showed 73% C-incorporation, were main degraders planted system, while Comamonadaceae involved lesser extent (about 64% C-incorporation). Among one key players could assigned Ralstonia pickettii. observed pathway occurred two subsequent monooxygenations aromatic ring. Our study provides suitable approach assess microbes complex ecosystems.