Comparative label-free LC-MS/MS analysis of colorectal adenocarcinoma and metastatic cells treated with 5-fluorouracil.

摘要: A label-free mass spectrometric strategy was used to examine the effect of 5-fluorouracil (5-FU) on primary and metastatic colon carcinoma cell lines, SW480 SW620, with without treatment. 5-FU is most common chemotherapeutic treatment for cancer. Pooled biological replicates were analyzed by nanoLC-MS/MS protein quantification determined via spectral counting. Phenotypic proteomic changes evident often similar in both lines. The SW620 cells more resistant treatment, an IC(50) 2.7-fold higher than that SW480. In addition, lines showed pronounced abundance pathways relating antioxidative stress response adhesion remodeling due For example, detoxification enzyme NQO1 increased while disparate members peroxiredoxin family, PRDX2 or PRDX5 PRDX6, elevated exposure either respectively. Cell adhesion-associated proteins CTNNB1 RhoA decreased expression differential quantitative proteomes these patient-matched drug underscores subtle molecular differences separating cancer cells.