5-Fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency
作者： K Bracht , A M Nicholls , Y Liu , W F Bodmer
关键词: Oncology 、 Fluorouracil 、 Methyltransferase 、 Antimetabolite 、 Genetics 、 Colorectal cancer 、 Mutation 、 Loss of heterozygosity 、 Internal medicine 、 Cancer 、 Replication (statistics) 、 Biology
摘要: Colorectal cancer is (CRC) one of the commonest cancers and its therapy still based on few drugs. Currently, no biological criteria are used to choose most effective established drugs for treatment. A panel 77 CRC cell lines was tested sensitivity 5-fluorouracil (5FU) using SRB assay. The responses were grouped into three categories correlated with genetic changes in lines. strongest clearcut correlation between response replication error status (mismatch repair deficiency). All other significant correlations (loss heterozygosity DCC mutations TGFbIIR) secondary association status. Our findings validate previous analyses mainly clinical data, indicate that could be a useful guide 5-fluorouracil-based therapy. Essentially, all previously described 5FU
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