From lithotroph- to organotroph-dominant: directional shift of microbial community in sulphidic tailings during phytostabilization

摘要: Engineering microbial diversity to enhance soil functions may improve the success of direct revegetation in sulphidic mine tailings. Therefore, it is essential explore how remediation and initial plant establishment can alter communities, and, which edaphic factors control these changes under field conditions. A long-term trial was established at a Pb-Zn-Cu tailings impoundment northwest Queensland. The amended and/or revegetated treatments were sampled from 3-year-old trial. In total, 24 samples examined using pyrosequencing 16S rRNA genes various chemical properties. results showed that positively controlled by soluble Si negatively S, total Fe As, implying pyrite weathering posed substantial stress on development All dominated typical extremophiles lithotrophs, typically Truepera, Thiobacillus, Rubrobacter; significant increases diversity, biomass frequency organotrophic genera (typically Nocardioides Altererythrobacter) detected treatment. We concluded appropriate phytostabilization options have potential drive community structure toward those natural soils, however, inherent environmental stressors limit such changes.