Differential α1-adrenoceptor labeling by []prazosin and []tamsulosin

摘要: The radioligand binding properties of [3H]prazosin and [3H]tamsulosin at alpha1-adrenoceptors several rat tissues, human prostate cloned alpha1-adrenoceptor subtypes were compared in Tris/EDTA buffer unless otherwise indicated. affinity tissue alpha1A- alpha1B-adrenoceptors was somewhat greater smaller, respectively, than that [3H]prazosin. In most tissues alpha1B-adrenoceptors, had a smaller Bmax Studies with liver showed this due to considerably poorer labeling agonist low sites, while both radioligands detected similar numbers high sites. Statistically significant differences the number sites for ligands not HEPES or glycylglycine buffer, as detectable receptor tended be greater, these buffer. Among labeled fewer alpha1B- but more alpha1D-adrenoceptors. We conclude do detect same under variety conditions. This should taken into account interpretation data obtained either radioligand.