COMPARATIVE EVALUATION OF ELISAS BASED ON TEN RECOMBINANT OR PURIFIED LEISHMANIA ANTIGENS FOR THE SERODIAGNOSIS OF MEDITERRANEAN VISCERAL LEISHMANIASIS
作者： IBTISSEM ABDMOULEH MAALEJ , MEHDI CHENIK , HECHMI LOUZIR , CHOKRI BAHLOUL , KOUSSAY DELLAGI
DOI: 10.4269/AJTMH.2003.68.312
关键词:
摘要: This study compared a panel of 10 enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis Mediterranean visceral leishmaniasis (MVL). The ELISAs were based on either one following Leishmania antigens: crude soluble antigens (SLAs), recombinant (r) (namely: rgp63, rK39, gene B protein, r H2A and H2B histones proteins, rLACK, rPSA-2, P20) purified lipophosphoglycan. Most test showed good performance (sensitivity > 85%, specificity 80%). rK39 SLA-based ELISA gave best results in terms sensitivity (100%) predictive value negative (100%). (97%) positive (92%) obtained with rK39. These show that several are suitable to design diagnostic MVL. However, proteins add little classic SLA, which still represents very less costly alternative.
ajtmh.org参考文章(45)
Roberto Badaró, Edgar M. Carvalho, Steven G. Reed, Immunofluorescent antibody test in American visceral leishmaniasis: sensitivity and specificity of different morphological forms of two Leishmania species. American Journal of Tropical Medicine and Hygiene. ,vol. 32, pp. 480- 484 ,(1983) , 10.4269/AJTMH.1983.32.480
P Natovitz, P Scott, E Pearce, A Sher, Vaccination against cutaneous leishmaniasis in a murine model. I. Induction of protective immunity with a soluble extract of promastigotes. Journal of Immunology. ,vol. 139, pp. 221- 227 ,(1987)
L Rolland-Burger, X Rolland, C W Grieve, L Monjour, Immunoblot analysis of the humoral immune response to Leishmania donovani infantum polypeptides in human visceral leishmaniasis. Journal of Clinical Microbiology. ,vol. 29, pp. 1429- 1435 ,(1991) , 10.1128/JCM.29.7.1429-1435.1991
P.J. Murray, T.W. Spithill, Variants of a Leishmania surface antigen derived from a multigenic family. Journal of Biological Chemistry. ,vol. 266, pp. 24477- 24484 ,(1991) , 10.1016/S0021-9258(18)54253-8
Paul G McKean, Katharine R. Trenholme, Desikan Rangarajan, Jane K Keen, Deborah F. Smith, DIVERSITY IN REPEAT-CONTAINING SURFACE PROTEINS OF LEISHMANIA MAJOR Molecular and Biochemical Parasitology. ,vol. 86, pp. 225- 235 ,(1997) , 10.1016/S0166-6851(97)00035-2
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Roberto Badaró, Gloria Orge, Steven G. Reed, Thomas C. Jones, Aldina Barral, Evaluation of the micro enzyme-linked immunosorbent assay (ELISA) for antibodies in American visceral leishmaniasis: antigen selection for detection of infection-specific responses. American Journal of Tropical Medicine and Hygiene. ,vol. 35, pp. 72- 78 ,(1986) , 10.4269/AJTMH.1986.35.72
Sara M. Debanne, Thomas M. Daniel, The serodiagnosis of tuberculosis and other mycobacterial diseases by enzyme-linked immunosorbent assay. The American review of respiratory disease. ,vol. 135, pp. 1137- 1151 ,(2015) , 10.1164/ARRD.1987.135.5.1137
A.Ben Salah, R.Ben Ismail, F. Amri, S. Chlif, F.Ben Rzig, H. Kharrat, H. Hadhri, M. Hassouna, K. Dellagi, Investigation of the spread of human visceral leishmaniasis in central Tunisia Transactions of the Royal Society of Tropical Medicine and Hygiene. ,vol. 94, pp. 382- 386 ,(2000) , 10.1016/S0035-9203(00)90112-3
T. G. Evans, M. J. Teixeira, I. T. McAuliffe, I. d. A. Barros Vasconcelos, A. W. Vasconcelos, A. de Queiroz Sousa, J. W. de Oliveira Lima, R. D. Pearson, Epidemiology of visceral leishmaniasis in northeast Brazil. The Journal of Infectious Diseases. ,vol. 166, pp. 1124- 1132 ,(1992) , 10.1093/INFDIS/166.5.1124