Immunofluorescent antibody test in American visceral leishmaniasis: sensitivity and specificity of different morphological forms of two Leishmania species.

摘要: This study was designed to determine which morphologic form and species of Leishmania is most suitable for detection antibody in sera from American visceral leishmaniasis (AVL) patients by the indirect fluorescent test (IFAT). Promastigotes amastigotes mexicana or donovani chagasi were used as sources antigen. A total 70 sera, including 30 AVL patients, healthy subjects 10 Chagas' disease study. Titers up a dilution 1:64 found with all four antigens. At titer 1:128, sensitivity IFAT using L. d. promastigotes source antigen 100% specificity at 1:32 98%. Although amastigote forms close similar titer, 32 91% only 80%. The promastigote also one that showed less cross reaction patients. Since reactivity between Trypanosoma cruzi well known serological tests, minimizing such critical importance diagnosis, we suggest should be choice diagnosis areas endemic trypanosomiasis.