Lysophosphatidic acid regulates adhesion molecules and enhances migration of human oral keratinocytes.

摘要: Oral keratinocytes are connected via cell-to-cell adhesions to protect underlying tissues from physical and bacterial damage. Lysophosphatidic acids (LPAs) a family of phospholipid mediators that have the ability regulate gene expression, cytoskeletal rearrangement, cytokine/chemokine secretion, which mediate proliferation, migration, differentiation. Several forms LPA found in saliva gingival crevicular fluid, but it is unknown how they affect human oral (HOK). The aim present study was therefore examine different expression adhesion molecules migration proliferation HOK. Keratinocytes were isolated biopsies obtained healthy donors challenged with LPA. Quantitative real-time RT-PCR, immunocytochemistry, flow cytometry used analyze molecules. Migration assays performed. strongly promoted E-cadherin occludin mRNAs translocation protein cytoplasm membrane. Occludin claudin-1 proteins up-regulated by HOK culture increased, reduced, addition This indicates can role regulation epithelial barrier increasing HOK, promotion inhibition proliferation.