Lysophosphatidic Acid Rescues Human Dental Pulp Cells from Ischemia-induced Apoptosis

摘要: Abstract Introduction Dental pulp is particularly susceptible to ischemic conditions (hypoxia and serum deprived) because it commonly exposed trauma, inflammation, chronic caries injury, pulpitis. We investigated the apoptotic response of human dental cells (HDPCs) varying levels oxygen mimic different degrees ischemia, tested whether lysophosphatidic acid (LPA) could reverse ischemia-induced apoptosis, possible mechanisms LPA. Methods HDPCs were cultured under mimicking deprivation ischemia for 2 days with or without LPA at 25 μg/mL. Flow cytometry JC-1 fluorescence used detect any change. Western blotting was measure expression apoptosis regulators B-cell lymphoma (Bcl-2) Bax, focal adhesion kinase (FAK), Src, extracellular signal-regulated (ERK), Akt. Results immunofluorescence showed that induce in treatment reduce cell death significantly. To clarify molecular mechanisms, blot results up-regulation both proapoptotic Bax antiapoptotic Bcl-2 during apoptosis. functioned as an cytokine by activation phosphorylation FAK ERK. No statistically significant difference found p-Src p-Akt. Conclusions A self-defense mechanism effectively rescue from via regulation phosphorylated a potent candidate biological therapy pulpal inflammatory diseases.