Flow cytometry analysis of transcription factors in T lymphocytes
作者： Diana I. Albu , Danielle Califano , Dorina Avram
DOI: 10.1007/978-1-60761-738-9_23
关键词:
摘要: Detection of transcription factors in immune cell populations, particularly subpopulations that are represented at low frequencies lymphoid and nonlymphoid organs, presents a particular challenge when using traditional methods such as western blot analysis. Therefore, development flow cytometry-based which allow identification specific populations is main interest. Here we developed optimized methodology for rapid convenient detection the factor BCL11B T lymphocyte cytometry. The optimal protocol employs saponin Tween 20 both during fixation permeabilization steps, demonstrate it efficient three anti-BCL11B antibodies covering distinctive epitopes. In addition, prove method preserves staining surface markers.
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