Histopathologic Alterations Associated with Global Gene Expression Due to Chronic Dietary TCDD Exposure in Juvenile Zebrafish

摘要: The goal of this project was to investigate the effects and possible developmental disease implication chronic dietary TCDD exposure on global gene expression anchored histopathologic analysis in juvenile zebrafish by functional genomic, analytic chemistry methods. Specifically, were fed Biodiet starter with added at 0, 0.1, 1, 10 100 ppb, fish sampled following 7, 14, 28 42 d after initiation exposure. accumulated a dose- time-dependent manner ppb caused accumulation female (15.49 ppb) male (18.04 post Dietary multiple lesions liver, kidney, intestine ovary dysregulation such as depletion glycogen retrobulbar edema, degeneration nasal neurosensory epithelium, underdevelopment intestine, diminution fraction ovarian follicles containing vitellogenic oocytes. Importantly, epithelium evidence endocrine disruption based alternatively spliced vasa transcripts are two novel significant results study. Microarray comparing vehicle control revealed dysregulated genes involved pathways associated cardiac necrosis/cell death, fibrosis, renal death liver death. These baseline toxicological provide for potential mechanisms dysfunctions induced biomarker disruption.