Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds.

摘要: Human airway smooth muscle (HASM) contraction plays a central role in regulating resistance both healthy and asthmatic bronchioles. In vitro studies that investigate the intricate mechanisms regulate this contractile process are predominantly conducted on tissue culture plastic, rigid, 2D geometry, unlike 3D microenvironment cells exposed to situ. It is increasingly apparent cellular characteristics responses altered between cultured substrates compared with topographies. Electrospinning an attractive method produce topographies for cell culturing as fibers produced have dimensions within nanometer range, similar cells' natural environment. We developed electrospun scaffold using nondegradable, nontoxic, polymer polyethylene terephthalate (PET) composed of uniaxially orientated nanofibers evaluated topography's effect HASM adhesion, alignment, morphology. The orientation provided contact guidance enabling formation fully aligned sheets muscle. Moreover, present elongated phenotype protein levels distribution. responded bronchoconstrictor bradykinin. platform presented provides novel model promotes development toward more vivo-like while providing topological cues ensure full alignment.