Heparan sulfate proteoglycan expression in human lung-cancer cells

摘要: Heparan sulfate (HS) functions as a co-factor in several signal-transduction systems that affect cellular growth, differentiation, adhesion and motility. HS, therefore, may also play role the malignant transformation of cells, tumor cell invasiveness formation metastases. To explore this hypothesis, we analyzed expression HS heparan proteoglycan (HSPG) histological sections human lung-cancer tissues assayed for presence HSPGs extracts lines, using panel native HS-, Δ-HS- HSPG (syndecan, glypican, CD44 perlecan) core protein–specific monoclonal antibodies. Compared to normal epithelia, non-small-cell lung carcinomas, particularly poorly differentiated tumors, often expressed reduced amounts major surface–associated (most consistently syndecan-1). or CD44-variant proteins, contrast, were found on all irrespective their differentiation. Perlecan, matrix-associated basement membrane bronchial epithelium, was undetectable invasive bronchogenic carcinomas. Staining reactions squamous-cell cells contact with stroma less areas these tumors. Reactions Δ-HS, however, not reduced, suggesting structural change cells. Poorly adenocarcinomas, yielded strong Δ-HS reactions. Marked differences observed among various carcinoma lines. Our results suggest tumors have markedly altered patterns expression, which contribute phenotype. Int. J. Cancer 74:335–345, 1997. © 1997 Wiley-Liss, Inc.