Analysis of dilute aqueous multifluorophoric mixtures using excitation–emission matrix fluorescence (EEMF) and total synchronous fluorescence (TSF) spectroscopy: A comparative evaluation

摘要: Excitation–emission matrix fluorescence (EEMF) and total synchronous (TSF) spectroscopy are two conceptually different techniques that have been used to map the responses of fluorophores present in a multifluorophoric mixture. EEMF was introduced four decades back most fluorimeters suitable computer program which allows acquisition spectra. Recently TSF has shown possess good application potential analytical fluorimetry started attracting attention chemists. data structure, however, is intrinsically from structure better understanding crucial utilising its potential. In work, comprehensive comparative study between spectroscopic set performed by taking aqueous mixtures containing low concentrations benzo[a]pyrene, chrysene, pyrene as test case. The structures were clearly explained an example. effects Rayleigh Raman scattering on quality sets studied. dilute subjected three chemometric PARAFAC, N-PLS, MCR-ALS analysis. particular found be highly attuned Obtained results analyses show provides more accurate spectral concentration information than sets. Therefore, could considered alternate for mixtures.