FINE STRUCTURE OF LISTERIA MONOCYTOGENES

摘要: Edwards, Mercedes R. (New York State Department of Health, Albany) and Roy W. Stevens. Fine structure Listeria monocytogenes. J. Bacteriol. 86:414-428. 1963.-Cells monocytogenes, at different stages growth, were fixed with osmium tetroxide treated uranyl acetate. The material was dehydrated in alcohol, embedded prepolymerized methacrylate, studied thin sections. In most the micrographs, plasmalemma (or plasma membrane) showed a pattern three dense lines, each ca. 25 A thick, alternating two light zones, 30 thick. outer zone regularly bridged by strands material, inner one not. line edge cytoplasm not always discernible because its similarity density ground cytoplasm, although it could be easily demonstrated lysed cells protoplasts. latter found to limited pair bounding core This corresponds "unit" membrane, but represents only part intact cell; therefore interpreted as being more complex than single unit membrane. Intracytoplasmic membranes various configurations clearly shown extensions plasmalemma. They may branch repeatedly anastomose form complicated honeycomb-like organelle or organelles appearances, sometimes lamellate. lamellar bodies are envisioned resulting from spiraled ingrowths. kinds ingrowths designated "plasmalemmosomes" indicate their origin; however, some these similar those described bacteria other authors. Plasmalemmosomes have been both aerobically anaerobically grown cells. Another outstanding feature many micrographs nucleoid, which contains fibrils measuring 50 diameter. These frequently appeared coiled order magnitude ascribed deoxyribonucleic acid molecules.