Excellence of the two-layer method (University of Wisconsin solution/perfluorochemical) in pancreas preservation before islet isolation☆

摘要: Abstract Background . In islet transplantation pancreatic preservation before isolation is an obstacle compromising yield and viability. We tested the feasibility of a two-layer method (University Wisconsin solution [UW]/perfluorochemical) for isolation. Methods Dog pancreases were processed into pure islets by Ricordi preceded five different preservations (groups 1-a 1-b, far 3 24 hours; groups 2-a 2-b, simple cold storage in UW group 3, without preservation). Islet yields functional success after autotransplantation liver compared among groups. Results Postpurification equivalents (IE)/gm pancreas rate 5600 (mean), 83% 1-a; 4000, 56% 1-b; 4700, 33% 2-a; 1300, 0% 2-b; 5000, 89% (p 0.2). Conclusions The more effective than conventional Clinical trials with are warranted.