MICROSATELLITE LOCI POLYMORPHISM IN RUSSIAN BLACK CURRANT (Ribes nigrum L.) VARIETIES FROM COLLECTION OF ALL-RUSSIAN RESEARCH INSTITUTE OF BREEDING FRUIT CROPS

摘要: Black currant is the main berry crop in Russia. The need to improve its assortment requires new effective methods be involved breeding. Application of DNA markers as a modern approach dealing with germplazm intensively used abroad works on black plants. We are first Russia who SSR studying gene pool. In this paper we report genotyping 27 accessions from collection All-Russian Research Institute Breeding Fruit Crops, including 16 varieties originated institute, 14 microsatellite loci. Electrophoresis 6 % denaturing PAAG followed by staining silver nitrate was for separation PCR products. All tested loci, except MS06g03, have been found polymorphic. On average 4.9 alleles were amplified per locus. Three fragments some at one locus that probably due duplication these loci genome accessions. A total 66 all rare frequency occurrence equal or less than 0.2. Eight unique found. observed heterozygosity ranged 0.259 (g2-H21) 1 (e4-D03) and averaged 0.608. Combinations allowed distinguish 3 (g2-H21, e1-O21) 15 (g2-G12) genotypes. minimum set four (e4-D03, g1-M07, g1-E03, g2-B20) accessions, i.e. multilocus profile has each sample. Pairwise genetic similarity coefficients 0.11 (between Odzhebin Sharovidnaya varieties) 0.95 Govtva Rtishchevskaya 0.346. Cluster analysis done. dendrogram shows few clusters high bootstrap support. Distribution between related consistent pedigrees most cases, but discrepancies data also Thus, Ocharovanie (1 1168 1⁄2 Ekzotika variety) had no common three (e4D03, g2-B20). It pollination other pollen error reproduction transplantation mutations Binar (Odzhebin Naryadnaya) two (g1-M07, g1-E03). Our results allow recommend evaluation domestic pool diversity, develop identification certification currant.